Evaluation of the Thin Agar Layer Oxyrase® Method for Recovery of Heat-Injured Foodborne Pathogens in Liquid Media and Food Systems

نویسندگان

  • Vivian CH Wu
  • Daniel YC Fung
چکیده

Cite this article: Wu VCH, Fung DYC (2014) Evaluation of the Thin Agar Layer Oxyrase® Method for Recovery of Heat-Injured Foodborne Pathogens in Liquid Media and Food Systems. J Hum Nutr Food Sci 2(2): 1027. *Corresponding author Vivian CH Wu, School of Food and Agriculture, Pathogenic Microbiology Laboratory, The University of Maine, 5395 Hitchner Hall, Orono, ME 04469-5735, USA, Tel: 1-207-5813101; Fax:1-2075811636; E-mail:

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Evaluation of thin agar layer method for recovery of acid-injured foodborne pathogens.

The thin agar layer (TAL) method of Kang and Fung was used to enumerate acid-injured foodborne pathogens. This method involves overlaying 14 ml of nonselective medium (tryptic soy agar [TSA]) onto a prepoured and solidified pathogen-specific, selective medium in a petri dish. After surface plating, injured cells resuscitated and grew on TSA during the first few hours of incubation; then, the se...

متن کامل

Recovery of heat-injured Listeria innocua.

Listeria innocua was subjected to thermal inactivation and the extent of heat-injured cells was quantified. Cultures were heated in liquid medium for different times, using temperatures in the range of 52.5 to 65.0 degrees C, and plated on Tryptic Soy Agar with 0.6% yeast extract (TSAYE) used as non-selective medium and on TSAYE plus 5% NaCl (TSAYE+NaCl) and Palcam agar with selective supplemen...

متن کامل

Application of thin agar layer method for recovery of injured Salmonella typhimurium.

Xylose lysine decarboxylase (XLD) medium, a selective plating medium, can inhibit heat-injured Salmonella typhimurium from growing, whereas tryptic soy agar (TSA), a nonselective medium, does not. To facilitate recovery of heat-injured S. typhimurium cells while providing selectivity of isolation of S. typhimurium from other bacteria in the sample, a thin agar layer (TAL) procedure was develope...

متن کامل

Agar underlay method for recovery of sublethally heat-injured bacteria.

A method of recovering sublethally heat-injured bacteria was developed. The procedure (termed the agar underlay method) uses a nonselective agar underlaid with a selective medium. In a two-chambered petri dish, the Lutri plate (LP), a nonselective agar is inoculated with a population of sublethally heat-injured bacteria. After a 2-h repair incubation period, selective agar is added to the botto...

متن کامل

Title : Effects of chilling methods for bacterial recovery and reducing bacteria on pork

To date, very little information exists on recovery methods for freeze-injured cells and the effect of blastor conventional-chilling for reducing specific pathogens associated with pork surfaces. To address these issues, two separate studies were conducted to determine 1) an effective recovery method for freeze-injured Listeria monocytogenes, Salmonella Typhimurium and Campylobacter coli associ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2015